| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070564 |
| Chromosome: | chromosome_8 |
| Location: | 3902112 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g378200 | PPO1 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCGAACGCGGCCTTGCCGGTGGCGGCCA |
| Internal bar code: | GGGCCGCTGCGTGGACGGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 55.3964 |
| LEAP-Seq n confirming: | 13068 |
| LEAP-Seq n nonconfirming: | 10522 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCACCCACATCTACCTC |
| Suggested primer 2: | GAGGGAGGAGAGGGAATGTC |