| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070590 |
| Chromosome: | chromosome 2 |
| Location: | 786316 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078858 | PSN1 | Presenilin protease; (1 of 1) K04505 - presenilin 1 (PSEN1, PS1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGGCAAGAACGGCGAGGGCCACGGAGA |
| Internal bar code: | TCTGGTCCCTCTAACAGCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 44 |
| LEAP-Seq percent confirming: | 43.0785 |
| LEAP-Seq n confirming: | 417 |
| LEAP-Seq n nonconfirming: | 551 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAAGATGTAGGCCACG |
| Suggested primer 2: | TCTTCACATCAAGGCACAGC |