| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070590 |
| Chromosome: | chromosome 3 |
| Location: | 3302281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166150 | (1 of 1) IPR015077 - Domain of unknown function DUF1858 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGACATCCCCGCTAACCGATGCTACCGA |
| Internal bar code: | ATGTTTACGGACAACCACTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 89.084 |
| LEAP-Seq n confirming: | 12225 |
| LEAP-Seq n nonconfirming: | 1498 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAATGCTAGTGCGGATG |
| Suggested primer 2: | TTCGAAGAGAGCTTGGGGTA |