| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070602 |
| Chromosome: | chromosome_4 |
| Location: | 2662943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g223100 | CAH1 | Carbonic anhydrase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCCGCACCATCAAGCTGGCCCGCTATCAC |
| Internal bar code: | CGGAATATCGGAACCTGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.3646 |
| LEAP-Seq n confirming: | 3284 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTGTAGCTACCTCGCTC |
| Suggested primer 2: | TTTGCACGTTTACTGATCGC |