Insertion junction: LMJ.RY0402.070604_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.070604
Chromosome:chromosome 1
Location:2084930
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g011150 (1 of 2) IPR000104//IPR011598 - Antifreeze protein, type I // Myc-type, basic helix-loop-helix (bHLH) domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTAGACATTGAGAGGTATTGAAGACATA
Internal bar code:TTTATCAGTAAGTGCCATTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:95.7983
LEAP-Seq n confirming:114
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGCTGTAAAGCCAGACG
Suggested primer 2:CAAACTTATCGCAACGCTCA