| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070613 |
| Chromosome: | chromosome 16 |
| Location: | 5181085 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACCGGAAAAGTAGACTAGGATGGGGTT |
| Internal bar code: | ATCATAGCGCCTTGCGTGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 54.8951 |
| LEAP-Seq n confirming: | 1099 |
| LEAP-Seq n nonconfirming: | 903 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGCTAGATGCTGCCTAC |
| Suggested primer 2: | CACTCTGGGCCTTGAGCTAC |