| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070624 |
| Chromosome: | chromosome 2 |
| Location: | 867525 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079150 | (1 of 1) PF03031//PF13671 - NLI interacting factor-like phosphatase (NIF) // AAA domain (AAA_33) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGGGGCCCAGGTTGGTGATAGAGGGCG |
| Internal bar code: | ACTTCACGGCACGTACATCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 95.6989 |
| LEAP-Seq n confirming: | 1068 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTTGGGCATCTACACCT |
| Suggested primer 2: | AGCCTCCGTAAAGCTGAACA |