| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070633 |
| Chromosome: | chromosome 1 |
| Location: | 4493330 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030600 | MAPKKK4 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGCTGACAGTCCTTGCGGCTGGCCGGC |
| Internal bar code: | ATCCAGTGGCGTTTAAGGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 21.0526 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 1215 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCTGGACTTACGGTCAT |
| Suggested primer 2: | TACCTGTGCTTCAACAAGCG |