| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070633 |
| Chromosome: | chromosome 5 |
| Location: | 1262832 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245352 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTCATGACCGCCCGCCCCCCCTCACGA |
| Internal bar code: | AGGCGACTGAAAAGGGGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 87.9202 |
| LEAP-Seq n confirming: | 2380 |
| LEAP-Seq n nonconfirming: | 327 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGTGATCAGGCCACACA |
| Suggested primer 2: | GCGAGCCGCTATCTACAATC |