Insertion junction: LMJ.RY0402.070639_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.070639
Chromosome:chromosome 3
Location:2472058
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g159550 (1 of 7) IPR011989//IPR016024 - Armadillo-like helical // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGACGTCGCGGCACAGACGACGAACACT
Internal bar code:TCCGCGGCCGATCGCTGCATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:217
LEAP-Seq percent confirming:99.8087
LEAP-Seq n confirming:8350
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTACTGTTGCAGGTGCT
Suggested primer 2:CAACAACCAATGGAGTGTGC