| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070643 |
| Chromosome: | chromosome 9 |
| Location: | 5642217 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401145 | (1 of 2) 3.1.4.12 - Sphingomyelin phosphodiesterase / Neutral sphingomyelinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTTGGGGAGTGGGGCGGAGGCGAGGGG |
| Internal bar code: | GACCCGAACCACGTGGGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 98.7194 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGATGCGAGTGAGAGA |
| Suggested primer 2: | CCCAAACTAAACCAAAGCCA |