| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070655 |
| Chromosome: | chromosome 14 |
| Location: | 423414 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610700 | PHC72 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCTGAGGTGGCGGCGGCAGCGGTGAGC |
| Internal bar code: | GGTGAGCGGTAGGCAGATGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 99.7323 |
| LEAP-Seq n confirming: | 745 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACACGAGTGCAGATGT |
| Suggested primer 2: | GCCTGTCCCTGTCTTCTGAC |