| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070662 |
| Chromosome: | chromosome 8 |
| Location: | 137620 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358538 | (1 of 1) K06443 - lycopene beta-cyclase (lcyB, crtL1, crtY) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTTACTAGAGGAGGTTGGGCTGCTGTA |
| Internal bar code: | CTTGAGCTTCCGCGGGGAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 63.205 |
| LEAP-Seq n confirming: | 1412 |
| LEAP-Seq n nonconfirming: | 822 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGGAAGGAAAAATGAGG |
| Suggested primer 2: | AGTTGCCGTGTCAACCCTAC |