| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070671 |
| Chromosome: | chromosome 7 |
| Location: | 4229934 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCGGGCGCTGCAGGCTTGGGGCGGCTT |
| Internal bar code: | GGGTGGTTAAGCGTTGAATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 372 |
| LEAP-Seq percent confirming: | 68.527 |
| LEAP-Seq n confirming: | 749 |
| LEAP-Seq n nonconfirming: | 344 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTTGCCAAAGCAGGATT |
| Suggested primer 2: | GTGTGCATGTGCATGTGTGT |