| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070695 |
| Chromosome: | chromosome 6 |
| Location: | 8885841 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311000 | FBT2 | (1 of 4) PTHR31585:SF0 - FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC; putative folate/pteridine transporter 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCAGTGACGGGTGAGCGAGCGGGCGGG |
| Internal bar code: | GAAGTTCCCATATGGGCCCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 97.8282 |
| LEAP-Seq n confirming: | 6171 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTGCCAAAATTGCCTAT |
| Suggested primer 2: | CTGCTCCAGGTGTCCTCAAT |