| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070718 |
| Chromosome: | chromosome 7 |
| Location: | 1908264 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325743 | GRX3 | (1 of 1) PTHR10293//PTHR10293:SF37 - GLUTAREDOXIN-RELATED PROTEIN // MONOTHIOL GLUTAREDOXIN-S14, CHLOROPLASTIC; Glutaredoxin 3, CGFS type, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGGCGGTGGTTCTGCCTCCCCCCTTTA |
| Internal bar code: | GCGTATTCGATCCTCGCTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 65.5392 |
| LEAP-Seq n confirming: | 1957 |
| LEAP-Seq n nonconfirming: | 1029 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTGCGCACGTTAAGCTC |
| Suggested primer 2: | AGGGCTACAAGGATGTGTGG |