| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070725 |
| Chromosome: | chromosome 3 |
| Location: | 3870068 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171050 | GHL1 | (1 of 2) 3.2.1.21 - Beta-glucosidase / Gentobiase; Glycosyl hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCGTACTTCGGCACACACGCGCGCACA |
| Internal bar code: | ATTCTGTTGCAGCGTGTAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 51.0467 |
| LEAP-Seq n confirming: | 3804 |
| LEAP-Seq n nonconfirming: | 3648 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACGACTCGAACTGGACT |
| Suggested primer 2: | ATGACTCCCACCCTTCTGTG |