| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070752 |
| Chromosome: | chromosome 1 |
| Location: | 529494 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002950 | (1 of 1) PF06677 - Sjogren's syndrome/scleroderma autoantigen 1 (Autoantigen p27) (Auto_anti-p27) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGAGGAGGAGGGGCAGCAGGCGGCCA |
| Internal bar code: | TCATGTGGATTTACAGTATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 99.5804 |
| LEAP-Seq n confirming: | 6407 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTACCGTTTTCCACAAT |
| Suggested primer 2: | TAGTGTCCACACGATCCCAA |