| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070793 |
| Chromosome: | chromosome 6 |
| Location: | 1743732 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGGTGCCCAGCCGGGCGGCCACACGCA |
| Internal bar code: | ACACCTTAGGTTCGCAAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 99.6818 |
| LEAP-Seq n confirming: | 4386 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTCTGAGGAGCTGGGT |
| Suggested primer 2: | CACAAATGTGTGGCTTCACC |