| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070797 |
| Chromosome: | chromosome 12 |
| Location: | 8682641 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546450 | (1 of 1) PTHR12472//PTHR12472:SF0 - RAB3-GAP REGULATORY DOMAIN // RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCGGGCTCGAGGACGGAGGAAGCACT |
| Internal bar code: | CTTTGCCGCTTCTTGGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGTTGGATAGGCCAGT |
| Suggested primer 2: | GTGCTGGACGTGGTATTGG |