| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070812 |
| Chromosome: | chromosome 9 |
| Location: | 5489192 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400330 | ALK6 | Aurora-like kinase; (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACATGCCTCATGACCCCTCCCACCTGA |
| Internal bar code: | GGCGAGAGTGGAGGTAACTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.0115 |
| LEAP-Seq n confirming: | 4307 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTATAGGCCTGGGGGTT |
| Suggested primer 2: | GCCTGTCTTCGTGTCCTCTC |