Insertion junction: LMJ.RY0402.070824_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.070824
Chromosome:chromosome 12
Location:8435021
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g548450 GT90-24,GT90F24 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 24 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAGGAGGACGAAGGGGCAGGAGAGAGAA
Internal bar code:TGGCACAATGAGTCCGCTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:900
LEAP-Seq percent confirming:77.3399
LEAP-Seq n confirming:157
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCGAAAGTGAGGAAGACC
Suggested primer 2:AAGGCGGGAAGAAAGAGAAG