| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070827 |
| Chromosome: | chromosome 4 |
| Location: | 606447 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217941 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCGTATGTGTGTGTGTGTGTGTGTGG |
| Internal bar code: | AGGTCGGCCCATGGCGGCCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 77.0053 |
| LEAP-Seq n confirming: | 432 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCGCTTCTCGTTTCTT |
| Suggested primer 2: | GTGGGTAGCCCTGCAAGTAG |