| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070838 |
| Chromosome: | chromosome 2 |
| Location: | 2334480 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090700 | MAPKKK8 | Mitogen-Activated Protein Kinase Kinase Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTAGACTGTAGATCGCCACAGGTGCGT |
| Internal bar code: | AGGAACAGGACACACCCGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.4192 |
| LEAP-Seq n confirming: | 2910 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGAGTTGCTGCTGTTG |
| Suggested primer 2: | CAGAGCTGAGGTAGGGGTTG |