| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070838 |
| Chromosome: | chromosome 13 |
| Location: | 938860 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568050 | SNRK2N | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACGACGCGCCGCCACTACGACCCTAAC |
| Internal bar code: | GATAACGAAAGTGTAGTCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 98.4317 |
| LEAP-Seq n confirming: | 1067 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACACAGCACAACTCAT |
| Suggested primer 2: | TTTGCCTAATGCAGCAAGTG |