| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070866 |
| Chromosome: | chromosome 16 |
| Location: | 4068983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669800 | (1 of 2) K04345 - protein kinase A (PKA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCCGTGGACCTCATCGACAAGCTGCTC |
| Internal bar code: | TGAACCCACTCACACTGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 97.7906 |
| LEAP-Seq n confirming: | 10977 |
| LEAP-Seq n nonconfirming: | 248 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAAAGTGCGTGTGTACG |
| Suggested primer 2: | ACCCTTGCACTCTCATCCAC |