| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.070871 |
| Chromosome: | chromosome 9 |
| Location: | 6649387 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408676 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGGATCCGGATTTGGATCCCAGCCCG |
| Internal bar code: | TCCTATCGTGCGTACCAGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 99.4503 |
| LEAP-Seq n confirming: | 2171 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCATCACGGGAACCTACG |
| Suggested primer 2: | GCAGTAGCTGACATGACGGA |