| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070874 |
| Chromosome: | chromosome 10 |
| Location: | 1070850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425300 | (1 of 4) 6.3.5.4 - Asparagine synthase (glutamine-hydrolyzing) / Glutamine-dependent asparagine synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACTGACTCTTGCGTTTGTTACAATTTG |
| Internal bar code: | CCGTTGGAAGCAGAGCCCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 38 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCGGAATATAGTTGGACC |
| Suggested primer 2: | CGTTCACACCCAATGAAGTG |