| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070890 |
| Chromosome: | chromosome 4 |
| Location: | 3913415 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230830 | (1 of 1) IPR000104//IPR001012//IPR029071 - Antifreeze protein, type I // UBX domain // Ubiquitin-related domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCAGGATGGCAGCGGGGTCCGCGCGCG |
| Internal bar code: | CAATTTTCACATTGGTTCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAACAAAACACCGATGGA |
| Suggested primer 2: | CACACACGTCTCTTCCCCTT |