| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.070895 |
| Chromosome: | chromosome_12 |
| Location: | 1190664 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g489750 | FAP52 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCGACCCCTTGATCTAAGCTTCCCCAAT |
| Internal bar code: | GAGGTCCTAACGAAGCCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 5.5355 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 785 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGATGGTGTCGTGTTGAG |
| Suggested primer 2: | CATTTTCACCATCAACGACG |