| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070916 |
| Chromosome: | chromosome 6 |
| Location: | 4838352 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278451 | PDK2 | Pyruvate dehydrogenase kinase; (1 of 2) 2.7.11.2 - [Pyruvate dehydrogenase (acetyl-transferring)] kinase / Pyruvate dehydrogenase kinase (phosphorylating) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGCCGTGAACTTGACGCTGTACACAGT |
| Internal bar code: | CACTCAATCGGGTGCGCCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 139 |
| LEAP-Seq percent confirming: | 55.9451 |
| LEAP-Seq n confirming: | 4404 |
| LEAP-Seq n nonconfirming: | 3468 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCCAGGCTTGTACTTGT |
| Suggested primer 2: | GAGTGGTAGGATCGCATGGT |