| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070916 |
| Chromosome: | chromosome 16 |
| Location: | 3442421 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670261 | (1 of 1) K15305 - vacuole morphology and inheritance protein 14 (VAC14, TAX1BP2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGAACCTCCGGACTAGGATTGCCTGT |
| Internal bar code: | GCTGTATTCAAGTCGGGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 69.2979 |
| LEAP-Seq n confirming: | 3178 |
| LEAP-Seq n nonconfirming: | 1408 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCGTAGACCTTTGTGGG |
| Suggested primer 2: | TACCAGCCCAAACTAAACCG |