| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070943 |
| Chromosome: | chromosome 4 |
| Location: | 812110 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217972 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCAGCTCTTGGATGCATCACTGGCAT |
| Internal bar code: | GTTTGGAATACAATGGACGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 48.2906 |
| LEAP-Seq n confirming: | 226 |
| LEAP-Seq n nonconfirming: | 242 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACGGTAACCAGTGAAGT |
| Suggested primer 2: | ATTGACAGCCAACACAGCAG |