| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070959 |
| Chromosome: | chromosome 12 |
| Location: | 7864187 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552850 | CGL77 | (1 of 2) 2.1.2.10 - Aminomethyltransferase / Tetrahydrofolate aminomethyltransferase; Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGTTGCATTGGAGAGAAACGCGTCCT |
| Internal bar code: | TTCGCACGCACCCGGATGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 50.6016 |
| LEAP-Seq n confirming: | 5383 |
| LEAP-Seq n nonconfirming: | 5255 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGGCTACCCATTCACT |
| Suggested primer 2: | AGAGCTAGGGGCCTTACTGC |