| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.070976 |
| Chromosome: | chromosome_7 |
| Location: | 3587429 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g336650 | MLH3 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTGCATGCTGCTCATCGTGCGCATTGCT |
| Internal bar code: | TGGGATAAGTGAAACGACAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 139 |
| LEAP-Seq percent confirming: | 54.0727 |
| LEAP-Seq n confirming: | 3379 |
| LEAP-Seq n nonconfirming: | 2870 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTCTGTATCGCTGCTG |
| Suggested primer 2: | GATGAAGAAAGCTTCGACGG |