| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.070999 |
| Chromosome: | chromosome 13 |
| Location: | 3740688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589426 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCCTTCTCGCCGGCGGCGGCAGCCTAA |
| Internal bar code: | TAATGGTGCATTTCCGTTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 97.4638 |
| LEAP-Seq n confirming: | 269 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGATACATTCCAATACGG |
| Suggested primer 2: | GGGCTCACAATTTCGTTTGT |