| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071000 |
| Chromosome: | chromosome 12 |
| Location: | 3693273 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514500 | RPS11 | Ribosomal protein S11, component of cytosolic 80S ribosome and 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAATGGCCACAGCTTGGCCTGGACTTGC |
| Internal bar code: | TGTACATGGCACCGTCATACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6 |
| LEAP-Seq percent confirming: | 95.8077 |
| LEAP-Seq n confirming: | 8113 |
| LEAP-Seq n nonconfirming: | 355 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACTTCTGCGACCCTTGC |
| Suggested primer 2: | TGGATAGGCTACCTGTTCCG |