| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071008 |
| Chromosome: | chromosome 12 |
| Location: | 4041965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517300 | (1 of 1) PF07738 - Sad1 / UNC-like C-terminal (Sad1_UNC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACGTGACTTGCGCGCTGAGGAAGAGTG |
| Internal bar code: | TGTCTTTCTGGATTAAATGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 455 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTCAGCACGTTCAGGTC |
| Suggested primer 2: | ACCTGAGCCTGCAGGAAGTA |