| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071018 |
| Chromosome: | chromosome 13 |
| Location: | 4214262 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592500 | (1 of 1) K00953 - FAD synthetase (FLAD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGCAGCGCCCAGCTCGAACCATGGC |
| Internal bar code: | ATGTCCGGCGGTCTTCTGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 22 |
| LEAP-Seq percent confirming: | 56.133 |
| LEAP-Seq n confirming: | 4169 |
| LEAP-Seq n nonconfirming: | 3258 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCGTGGTAGGTCCAGTC |
| Suggested primer 2: | CCGTTACCGTTTTGCTCATT |