| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071041 |
| Chromosome: | chromosome 17 |
| Location: | 3105414 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721250 | QilinDyf-3,FAP22,IFT38,Cluap1 | (1 of 1) PF10234 - Clusterin-associated protein-1 (Cluap1); Intraflagellar Transport Protein 38 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCTGAAACTGACGTCTGACCGGCATCA |
| Internal bar code: | GATTCCTTGTTATGATCAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 93.9463 |
| LEAP-Seq n confirming: | 4547 |
| LEAP-Seq n nonconfirming: | 293 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTATCCAAGTCCGCTGC |
| Suggested primer 2: | AGGATGACGACGAGGATGAC |