Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.071059 |
Chromosome: | chromosome 13 |
Location: | 3651518 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g588736 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGGGGCAGCGGAGGGTTGCATGTGCC |
Internal bar code: | GGCTCGTTTTCTGATGGCGTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 886 |
LEAP-Seq percent confirming: | 99.1545 |
LEAP-Seq n confirming: | 1290 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTTTGGCGACTCTTCACAG |
Suggested primer 2: | CACATCTTGGTAGCGGTCCT |