| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.071092 |
| Chromosome: | chromosome 17 |
| Location: | 860121 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702400 | PUS5 | (1 of 1) 3.5.4.26//5.4.99.28 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase // tRNA pseudouridine(32) synthase / Pseudouridine synthase RluA; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTTGGGCAACATGAGGGCCCGCTACCA |
| Internal bar code: | TGGTGGTAAAATCGAGGGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 924 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCCTATGTGCCAAGTT |
| Suggested primer 2: | AGCGCTACCTGCAGCTCTAC |