Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.071127 |
Chromosome: | chromosome_14 |
Location: | 2735462 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g626900 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGAGGAGGGATGCCCTTCATGTGGGTCG |
Internal bar code: | GGTACTACGCCGGCTGGCCGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 171 |
LEAP-Seq percent confirming: | 98.2055 |
LEAP-Seq n confirming: | 3010 |
LEAP-Seq n nonconfirming: | 55 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGATCTCGGTGCACTGCTT |
Suggested primer 2: | GTTCCCCCATCATCATCATC |