Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.071137 |
Chromosome: | chromosome_12 |
Location: | 3429042 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g496500 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCATGCGTGGGCAGAGACGTTGGCGTTGGA |
Internal bar code: | CGCAGGGACGACATGCGAACGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 59 |
LEAP-Seq percent confirming: | 99.4186 |
LEAP-Seq n confirming: | 171 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGTGCTGCAAATAGATTGA |
Suggested primer 2: | AAATCTCCCACCTGCATACG |