| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.071147 |
| Chromosome: | chromosome 3 |
| Location: | 52335 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143747 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAGTCCGGGAGAACCATCCTACGTTCA |
| Internal bar code: | GTCTCGTGTAATAGTACTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 99.0476 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATGATGTCGATGGGGTT |
| Suggested primer 2: | GGGGTATCACTGCAGTTCGT |