Insertion junction: LMJ.RY0402.071159_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.071159
Chromosome:chromosome 2
Location:4343257
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g099350 SRR8 Scavenger receptor cysteine rich (SRCR) protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGATGCGCCGGCCAGCCGCTGGCTTGGG
Internal bar code:CTCCGGTTTCCCTCTTACATT

Confirmation - LEAP-Seq

LEAP-Seq distance:883
LEAP-Seq percent confirming:99.7651
LEAP-Seq n confirming:2548
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCCAGGGTTGATAACAT
Suggested primer 2:GGATCAAGACGGATCAAGGA