| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.071165 |
| Chromosome: | chromosome 16 |
| Location: | 4187789 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668750 | MTA5 | Hypothetical protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCCTGGCGCCGCCACAGCCGCCGCCA |
| Internal bar code: | GCAGCGAACGTGAAGGGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 99.1124 |
| LEAP-Seq n confirming: | 335 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGATGTGCAAGTGAATG |
| Suggested primer 2: | GCTGCCTCTTGGTCACAGAT |