| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.071181 |
| Chromosome: | chromosome 16 |
| Location: | 2590280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661588 | OPR102 | OctotricoPeptide Repeat Protein 102 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTTCCAGGTGGGTGCCCAGCGGTGCA |
| Internal bar code: | CCCAGTTGCAAAGGAGGGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 98.4988 |
| LEAP-Seq n confirming: | 853 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAATGCAGACGACATCCTG |
| Suggested primer 2: | AGTACGGTATGATGGGCGAG |