Insertion junction: LMJ.RY0402.071186_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.071186
Chromosome:chromosome 2
Location:1452505
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g083750 ROC75 (1 of 1) IPR006447//IPR009057//IPR017930 - Myb domain, plants // Homeodomain-like // Myb domain; Rhythm Of Chloroplast 75 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATAAGCCACGATAACCTATCTCTTCCGC
Internal bar code:AGCATGACGGCTTGATAGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:337
LEAP-Seq percent confirming:99.7768
LEAP-Seq n confirming:447
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCGCAGAAACCTAGCAG
Suggested primer 2:ACGCACACACACACACACAC