Insertion junction: LMJ.RY0402.071204_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.071204
Chromosome:chromosome 16
Location:703317
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g691350 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCAGAAGGCTCACCCGCAAGTTCCAGT
Internal bar code:TCACTGGAACAGGAAGGCAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:61
LEAP-Seq percent confirming:18.035
LEAP-Seq n confirming:268
LEAP-Seq n nonconfirming:1218
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTACATGGTCAATGCGAACG
Suggested primer 2:ACTGCTGGAATTGGAACCAC